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KMID : 0363420180320040071
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Journal of Korean Oriental Pediatrics
2018 Volume.32 No. 4 p.71 ~ p.86
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The Effects of Gardenia jasminoides J.Ellis on Allergic Inflammation in RBL-2H3 Mast Cells and OVA/alum-sensitized Mice
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Kim Kyoung-Ri
Lee Jin-Hwa
Kim Eun-Jin
Kim Yun-Hee
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Abstract
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Objectives: The purpose of this study is to investigate the effects of Gardenia jasminoides for. grandiflora extracts’ (GAJ) anti-inflammatory effect on RBL-2H3 mast cells and OVA/alum-sensitized mice.
Methods: In this study, IL-4 and IL-13 production was measured via ELISA analysis, and mRNA expressions of GM-CSF, IL-4, IL-5, TNF-α, IL-6 were analyzed by real-time PCR. In addition, MAPKs and NF-κB p65 transcription factors were examined using western blotting, and ELISA was used to understand IgE, IL-4, and IL-13 production in ovalbumin-allergic mice in in vitro study.
Results: As a result of this study, 1. GAJ were observed to suppress the mRNA expression of GM-CSF, IL-4, IL-13, IL-5, TNF-α, IL-6 in comparison to PMA 50 ng/㎖, ionomycin 0.5 μM (PI) control group. 2. GAJ also inhibited the IL-4, IL-13 production in comparison to PI control group. 3. Western blot analysis showed decrease on the expression of mast-cell-specific transcription factors, including MAKPs (ERK, JNK, p38) and NF-κB p65. 4. Orally-administered GAJ group in OVA/alum induced Balb/c mice showed decreased level of OVA-specific IgE in the serum. This group also has shown decreased the level of IL-4, IL-13 in the splenocyte culture supernatant.
Conclusions Obtained results suggest that GAJ may regulate the allergic inflammation by transcription factors MAKPs (ERK, JNK, p38) and NF-κB p65 causing inhibition of Th2 cytokines in mast cells and OVA/alum-sensitized mice.
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KEYWORD
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Gardenia jasminoides for. grandiflora extracts (梔子), RBL-2H3 mast cell, OVA/alum-sensitized mice, Th2 cytokines, Balb/c mice, Allergic inflammation
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